Influence of Harvesting Season, Leaf Position and Wilting Period on Essential Oil Content of Eucalyptus globulus Leaves

摘要

The aim of this study is to determine the essential oil content variation at harvesting day (EOC0) andat wilting day (EOCw) of Eucalyptus globulus leaves was evaluated as independent factors that are harvestingseason (Long Rain season and Dry Season), the leafs type (Top, middle and bottom) and wilting period from0 h (W0) to 96 h (W4) were selected in to the experiment. A completely randomized design with three replicationswas used in the experiment. The oil was obtained by hydrodistillation of Eucalyptus leaves using Clevengerapparatus for 3 h. The distillate oil was measured and the essential oil content was expressed in percent basedon harvesting day and wilting day calculations. EOC0 and EOCw of E. globules were highly significantly(p0.001) affected by harvesting season, leaf position and wilting period. Interaction effect of harvestingseason, leaf position and wilting period on essential oil content at harvesting day and wilting period ofE.globulus is highly significant (p0.0001) in higher yielding of the EOC0and EOCw were found at wilting period72 h (W3) and 96 h (W4) with long rain season and dry season according to the respective value of EOC0 andEOCw (1.58% and 3.13%), respectively. The smallest essential oil content EOC0 (0.93%) and EOCw (0.94%) wasobtained for samples harvested during long rain season from bottom part distilled without wilting (W0). In viewof the results, it can be concluded that the day four wilting period and top leaf part are the most advantageousfor a maximum of essential oil recovered when the globulus leaves were distilled.