A monoclonal blocking ELISA for the serological diagnosis of bovine herpesvirus type 1 (BHV-1) infections

摘要

.- Teixeira M.F.B., Esteves, P.A, Schmidt, C.S., Dotta M.A. & Roehe, P.M. 2001. [A monoclonal blocking ELISA for the serological diagnosis of bovine herpesvirus type 1 (BHV-1) infections] ELISA de bloqueio monoclonal para o diagn?3stico sorol?3gico de infec?§?μes pelo herpesv?-rus bovino tipo 1 (BHV-1). Pesquisa Veterin??ria Brasileira 21(1):33-37. Centro de Pesquisa Veterin??ria Desid??rio Finamor (CPVDF), FEPAGRO, Cx. Postal 2076, Porto Alegre, RS 90001-970, Brazil. A monoclonal antibody-based blocking enzyme linked immunosorbent assay (ELISA-M) was developed and standardized for the detection of antibodies to infectious bovine rhinotracheitis virus (Bovine Herpesvirus type 1; BHV-1). A total of 266 samples of bovine sera (148 negative and 118 positive) were tested and compared with the results of a standard serum neutralization (SN) test. The ELISA-M was adjusted to 92.37% sensitivity, 92.56% especificity, 93.83% negative predictive value, 90.83% positive predictive value and to an accuracy of 92.48%, with an agreement index (k) equal to 0.85. The main advantages presented by the ELISA-M were its practicality and rapidity in performance. This test was shown to be a suitable alternative to SN tests in the detection of BHV-1 antibodies in cattle. However, the ELISA was unable to discriminate between BHV-1 and bovine herpesvirus type 5 (BHV-5) antibodies. INDEX