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Molecular identification of Cucumber mosaic virus isolates of subgroup IB associated with mosaic disease of eggplant in India

机译:印度茄子花叶病相​​关的IB亚群黄瓜花叶病毒的分子鉴定

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摘要

Association of Cucumber mosaic virus (CMV) with severe mosaic disease of eggplant (Solanum melongena L.) collected from Lucknow and Kanpur, India was initially detected by host reaction and serological assay and confirmed by RT-PCR employing coat protein gene specific primers. Further, molecular identification of the virus isolates was done by cloning and sequence analysis of the complete RNA3 genome. Based on 97–99?% identities and phylogenetic relationships, the virus isolates infecting eggplant were identified as members of CMV subgroup IB.
机译:黄瓜花叶病毒(CMV)与从印度勒克瑙和坎普尔采集的茄子(Solanum melongena L.)严重花叶病的关联最初是通过宿主反应和血清学检测发现的,并通过使用外壳蛋白基因特异性引物的RT-PCR进行了证实。此外,通过克隆和完整RNA3基因组的序列分析完成了病毒分离株的分子鉴定。基于97-99%的身份和系统发育关系,感染茄子的病毒分离株被鉴定为CMV IB亚组成员。

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