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首页> 外文期刊>Turkish Journal of Fisheries & Aquatic Sciences >Optimization of Thermal Shock for Poliplody Induction in Rainbow Trout (Oncorhynchus mykiss) under Photoperiodic Control of Spawning
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Optimization of Thermal Shock for Poliplody Induction in Rainbow Trout (Oncorhynchus mykiss) under Photoperiodic Control of Spawning

机译:产卵光周期控制下虹鳟(Oncorhynchus mykiss)感病诱导的热冲击优化

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摘要

Natural fish stocks could be at risk due to interbreeding or competition with farmed fish, which often differ genetically from their counterparts in wild. Farming of sterile or all-female triploids could be a powerfull preventative measure for preservation of natural gene resources or biodiversity of native species. Although, techniques for ploidy are well described in many fish species, there is no report whether the previous techniques applicable in out-off-season production induced by artificial photoperiod that is widely used in salmonid aquaculture. In order to optimize and refund ploidization protocol for off-season production of diploid gynogenomes and triploids three experiments were conducted to determine the optimal UV inactivation of sperm and heat shock treatment of eggs in rainbow trout (Oncorhynchus mykiss). High survival at yolk sac absorption stage (82.3–84.5%) and high yields (approx. 100%) were achieved from the range of treatments applied. There were significant differences in survival rates among treatments and the best optimisation was defined as the shock timing at 20 minutes post-fertilisation for 10 min duration at the shock temperature of 26 °C.
机译:天然鱼类种群可能由于与养殖鱼类的杂交或竞争而处于危险之中,而遗传上通常与野生鱼类不同。不育或全雌三倍体的养殖可能是保存天然基因资源或本地物种生物多样性的有力预防措施。尽管在许多鱼类中已经很好地描述了倍性技术,但尚无关于以前的技术是否适用于鲑鱼养殖中广泛使用的由人工光周期引起的淡季生产的报道。为了优化和退还二倍体雌激素组和三倍体的淡季生产的倍性协议,进行了三个实验,以确定虹鳟鱼(Oncorhynchus mykiss)精子的最佳紫外线灭活和鸡蛋的热激处理。卵黄囊吸收期的高存活率(82.3–84.5%)和高产率(约100%)可从所应用的治疗范围中获得。各治疗之间的存活率存在显着差异,最佳的优化定义为受精后20分钟,在26°C的冲击温度下持续10分钟的冲击时间。

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