Multichannel spectrophotometry for analysis of organophosphate paraoxon in beverages

摘要

Multichannel spectrophotometry was performed to assay forparaoxon in spiked beverages. A 96-well microplate was used for thispurpose. The measuring protocol was based on inhibition of the enzymeacetylcholinesterase by paraoxon that resulted in decreased or noreaction of the enzyme product thiocholine with Ellman's reagent(5,5'-dithiobis [2-nitrobenzoic acid]). The above assay waspractically tested using spiked drinking water, mineral water, andcoffee. Analytical parameters such as the limit of detection, time,and sample size consumption were adequate. The limit of detection forbeverages ranged from 32 to 48 ppb corresponding to 0.32-0.48 ng ofparaoxon in absolute values. The described assay seems to beconvenient in terms of practical use.