Development and In-House Validation of HACH Spectrophotometry Method for Determination of Phosphoric Acid In Cola Beverages

摘要

A cheap and a rapid HACH spectrophotometric method 8048 was developed and validated for the quantitative estimation of phosphoric acid in soft drinks. The developed method resulted in phosphoric acid exhibiting linearity in the range from 0.02 to 2.5 mg/L. The precision is exemplified by relative standard deviation of 3.45%. Percentage Mean recovery was found to be in the range of 90-107%, during accuracy studies. The limit of detection (LOD) and limit of quantitation (LOQ) were found to be 0.0075 mg/L and 0.02 mg/L respectively. Accordingly, it is concluded that the developed HACH spectrophotometric method 8048 is accurate, precise, linear and rugged, and therefore the method can be used for the routine analysis of phosphoric acid in soft drinks.