Up-regulation of corticotropin releasing hormone is associated with the downregulation of corticotropin releasing hormone binding protein and up-regulation of IL-33 and IL-8 ex pression in psoriasis

摘要

Purpose: To determine the ex pression of corticotropin releasing hormone (CRH) in psoriasis and normal skin biopsy samples, and to correlate the ex pression of CRH with the ex pression of CRHBP and inflammatory cytokines IL-8 and IL-33. Methods: Psoriasis and normal skin biopsy samples were obtained from three psoriatic and three normal healthy patients. The mRNA ex pression was examined by quantitative real-time polymerase chain reaction (RT-PCR). Protein ex pression analysis was carried out by Western blotting and then further validated by immunohistochemistry. Results: The results of the present study revealed that the ex pression of CRH was highly significant (p 0.0001) in psoriatic skin, compared to normal skin. Increase of CRH in psoriatic samples ranged from 2.7 to 3.5-fold. ex pression of CRH was associated with the concomitant downregulation of CRHBP in all the psoriatic skin biopsy samples, with ex pression of CRHBP 3.0 to 6.2-fold lower in psoriatic skin than in normal skin. Analysis of mRNA ex pression of IL-8 and IL-33, revealed that ex pression of both IL-8 and IL-33 was significantly (p 0.0001) upregulated in psoriatic skin samples while the ex pression of IL-8 and IL-33 was approximately 4.1- and 3.2-fold higher in psoriatic skin than in normal skin. The ex pression of CRHBP, IL-8 and IL-33 was further confirmed by western blotting and immunohistochemistry results. Conclusion: The results confirm that the ex pression of CRH is associated with the suppression of CRHBP and upregulation of IL-8 and IL-33.