Purpose: To investigate the anti-osteoporosis effect of Astragalus membranaceus (Fisch.) Bunge. extract (AMBE) in experimental rats. Method: Female Sprague-Dawley rats were randomly divided into six groups: control group, ovariectomy (OVX) with vehicle group, OVX with 17β-estradiol (E2, 25 μg/kg/day) group, and OVX with AMBE doses (60, 120 and 240 mg/kg/day) groups. Daily oral administration of AMBE or E2 was started 4 weeks after OVX and lasted for 16 weeks. The bone mineral density (BMD) of L4 vertebrae and right femurs was evaluated. The length of each femur was measured with a micrometer, and the center of diaphysis was determined. Three representative L4 vertebrae were selected to evaluate trabecular microarchitecture. Serum alkaline phosphatase (ALP), urinary calcium (U-Ca), urinary phosphorus (UP), urinary creatinine (Cr) and osteocalcin (OC) levels were measured. Results: AMBE dose-dependently inhibited the bone mineral density (BMD) reduction of L4 vertebrae (0.27 ± 0.03 g/cm2, p 2, p Conclusion: The findings of this study indicate that AMBE prevents OVX-induced osteoporosis in rats.
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机译：目的：研究黄芪的抗骨质疏松作用。提取物（AMBE）在实验大鼠中。方法：将雌性Sprague-Dawley大鼠随机分为六组：对照组，带载体的卵巢切除术（OVX）组，带17β-雌二醇（E2，25μg/ kg / day）的OVX组和带AMBE剂量的OVX（60， 120和240 mg / kg /天）。 OVX后4周开始每日口服AMBE或E2，持续16周。评估L4椎骨和右股骨的骨矿物质密度（BMD）。用千分尺测量每个股骨的长度，并确定骨干的中心。选择了三个代表性的L4椎骨来评估小梁微结构。测量血清碱性磷酸酶（ALP），尿钙（U-Ca），尿磷（UP），尿肌酐（Cr）和骨钙蛋白（OC）水平。结果：AMBE剂量依赖性地抑制了L4椎骨的骨矿物质密度（BMD）降低（0.27±0.03 g / cm 2 ，p 2 ，p结论：本研究的结果表明AMBE可预防OVX诱发的大鼠骨质疏松症。
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