High-Yield Laccase-Producing Strains Constructed by Protoplast FusionBetween Bacterium and Fungus

摘要

The construction of high-yield laccase-producing fusant was achieved by inter-kingdom protoplast fusionbetween Pleurotus ostreatus and Escherichia coli. The optimized protoplasts formation and regeneration conditionsdemonstrated that with the presence of 1.5% cellulase +1.0% snailase and 0.6M mannitol at 30°C for 3h. The fusants werescreened by different characters between two parental strains and further identified by laccase activity. We obtain one ofthe genetically stable fusants, Strain F. The fusant F could produce the highest yield of laccase, about 22% higher thanthat of the parental strain. The results suggest protoplast fusion technique may be considered as a promising technique inenvironmental pollution control.