The Natural Product NI-07, Is Effective Against Breast Cancer Cells WhileShowing No Cytotoxicity to Normal Cells

摘要

With the exception of a few anticancer agents, most breast cancer chemotherapeutics currently used havedevastating effects on normal cells. We investigated the effectiveness of the natural product NI-07, derived from Arctiumlappa,to determine its killing potential on breast cancer cells and its cytotoxic effects in breast normal cells. The breastcancer cell lines HCC1419, MCF7, MDA-MB-231, MDA-MB-468, SKBR3, the normal mammary epithelial cell lineHME 50 HT and the normal mammary fibroblast cell line CCD-1074sk were analyzed in concurrently treated cultures ofNI-07, Taxol? or Untreated. NI-07- treated and Taxol? -treated cells were cultured for two weeks to assess cellresistance/recovery. Cell viability was determined by cell counts, Trypan Blue exclusion and microscopy. Additionally,cell viability and cytotoxicity were measured by XTT. Statistical significance (p ≤ 0.05) of NI-07 to Untreated or Taxol?was first determined using Two-Way ANOVA. Identified significant differences were further analyzed by One-WayANOVA and Tukey's test for honestly significant differences. The effect size of NI-07 compared to untreated or Taxol?was determined using Cohen's d. Our results showed significant declines in cell viability occurring in NI-07- treatedcancer cells after 48 hours of treatment in contrast to the more rapid effect of Taxol? (<24h). Additionally, cancer cellrecovery was less effective in NI-07 versus Taxol?. Furthermore, NI-07 showed no cytotoxicity in normal cells. Thislack of cytotoxicity, coupled with its killing efficiency in cancer cells, suggests that NI-07 could potentially make a stronganti-cancer compound or neo-adjuvant to current chemotherapy-based treatments.