VITAMIN-D DEFICIENCY; Assessment of potential risk factor and Antioxidative status in Vitamin-D deficient females.

摘要

Background: Deficiency of vitamin D is characterized by the low bone masswhich leads to the bone fragility and high risk of fractures. Bone fractures causes the formationof free radicals, generated by the tissue damaged. Uncontrolled production of free radicalsaccelerates the oxidative stress and increased the bone remodeling process ultimatelycauses osteoporosis. One of the most damaging effects of free radicals is lipid peroxidation;end product of which is MDA, it also act as major factor in osteoblastic activity. Low level ofantioxidative defense system found in osteoporotic patients due to the deficiency of vitamin D.Many important mineral ions removed from bones and risk of bone fragility increases. Currentstudy is aim to check the antioxidative effect produced from excess reactive oxygen speciescompared with low level of vitamin D which is held responsible for higher or lower activityof bone cells. Study Design: Case Control Study. Setting: Study was conducted at Instituteof Molecular Biology and Biotechnology (IMBB), University of Lahore. Period of Study: Oneyear. Materials and Methods: Blood samples of 272 post-menopausal osteoporotic womenbetween the age 49-57 were collected from Jinnah hospital Lahore. While the samples of 92individuals were served as a control. Concentration of both enzymatic and non-enzymaticantioxidant such as CAT, GSH, SOD, GPx and GR, vitamin A, C and E and levels of MDAwere estimated spectrophotometrically. While the concentration of IL6, AOPPS, AGEs, TNF-α,MMP9, Isoprostanes, LDH, cholesterol, triglycerides, free fatty acids and phospholipid weremeasured by using commercially available Elisa kits. Results: Blood plasma levels of vitaminD were significantly lower in osteoporosis patients than in normal subjects. In addition, levelof stress biomarker such as MDA was found to be higher in patients as compared to control.Due to oxidative stress, level of antioxidants (GSH, CAT, and SOD) was found to be reduced.Blood cells and many other important minerals are also reduces in patient group from theirnormal amount. Conclusion: It concludes that excess production of free radicals over whelmsthe antioxidative system, thus it may leads to osteoporosis. Further more antioxidant speciessubjected to body might protect bone loss and also help in acceleration of healing of fracturedbones.