Brain Damages by Tripanossoma cruzi Increased Blood Brain BarrierPermeability in Holtzman Rats: Blood Brain Barrier Permeability in Chagas'Disease

摘要

The T. cruzi strains are a complex of multi-clonal populations that affects the brain with more severity inchildren or young animals, suggesting that functional immaturity of the blood brain barrier (BBB) might be involved in T.cruzi infection in the brain. This study investigated relationships between BBB permeability and T. cruzi infection in therat brain. The animals were inoculated with one of 3 strains of T. cruzi at 30 days of age, and BBB permeability wasincreased by subcutaneous kainic acid injection in half of the infected animals. The animals were sacrificed at 13 days ofinfection. The brain sections were stained with hematoxylin and eosin. The all brains of the infected animals with andwithout kainic acid showed both glial nodules and perivascular infiltrates due to T. cruzi infection. Number of these glialnodules was counted in each rat, and statistical comparison indicated that kainic acid-treatment significantly increasedmean numbers of glial nodules in all of the rats infected with the 3 strains. The results indicated that the increased BBBpermeability was one of the factors for facilitation of the entry of T. cruzi into the brain.