Detection of H. pylori in gastric biopsies: A cost effective method for developing countries.

摘要

H. pylori is the most common organism implicated in the pathogenesis of chronicgastritis. The aim of this study is to assess the reliability and cost effectiveness of hematoxylinand eosin (H&E) stain for the detection of H. pylori in gastric biopsies for developing countrieswith immunohistochemical markers (IHC) as gold standard. Study Design: Descriptive crosssectionalstudy. Setting: OPD of Kuwait teaching hospital and Mercy teaching hospital. Period:April 2017 to December 2017. Materials and Methods: 50 consecutive endoscopic gastricbiopsies collected according to the Updated Sydney Protocol from patients with dyspepticsymptoms. The data was entered and analyzed in SPSS version 19. Sensitivity, specificity,positive predictive value, and negative predictive value were calculated for each stainingtechnique. Chi square test was used for calculating p value. A value less than or equal to0.05 was considered statistically significant. Results: The most common presenting symptomwas epigastric pain, nausea/vomiting and bloating/burping. According to Updated SydneyProtocol, the most common diagnosis was mild chronic inflammation. Sensitivity, specificity, positive predictive and negative predictive value of H&E was 46.8 %, 88.8%, 88.2% and 48.5%respectively. Conclusion: Our study concludes that used alone H&E can easily detect H. pyloriin gastric biopsies in most of the cases. However, IHC may be used in selected patients withnegative H&E results and strong clinical suspicion of H. pylori infection.