Antidiabetic effect of withanolides and liraglutide on serum insulin level and pancreatic histology in diabetic rats.

摘要

Objectives: Type 2 diabetes is characterized by hyperglycemia and occurs asa result of insulin resistance and pancreatic beta cells failure to compensate. Present studywas conducted to determine the antidiabetic effect of Withania coagulans and liraglutide onpostprandial serum insulin levels and pancreatic histological features in streptozotocin induceddiabetic rat. Study Design: This Randomized Control Trial. Setting: At multidisciplinary labIslamic International Medical College, Rawalpindi in collaboration with Animal House, NationalInstitute of Health, Islamabad. Period: From March 2016 to April 2017. Material and Methods:Forty male Sprague daily rats were randomly divided into normal Control Group A (n=10) andExperimental Group (n=30). Experimental group was given streptozotocin (30mg/kg/day)intraperitoneally for 5 days and diabetes was confirmed in experimental group by measuringfasting blood glucose level (mg/dl). Experimental group was divided further into Group B(Diabetic control), Group C (Withania coagulans treated) and Group D (Liraglutide treated).Group C were given Withania coagulans in addition to normal diet and Group D receivedLiraglutide besides normal diet for 30 days. Second sampling for evaluating postprandial seruminsulin level and pancreatic histology was done after 30 days. Result: Postprandial serum insulinlevels of Withania coagulans treated Group C (5.54 + 0.23 μU/ml) and Liraglutide treated GroupD (6.06 + 0.17 μU/ml) were significantly raised in comparison with Diabetic control Group B(3.50 + 0.19 μU/ml), whereas islets of Langerhans cell morphology were markedly improvedin Group C and D as compared to Group B. Conclusion: Withania coagulans significantlyincreases postprandial serum insulin level and improves pancreatic beta cells architecture.