The correlation between percent liver weight (PLW) and percent fat cells (PFC) of liver in HFD-STZ induced diabetic rats in comparison with insulin, metformin and combination treated diabetic rats.

摘要

Background: The study was designed to evaluate short term effects ofcommonly used antidiabetic drugs on liver. The objective of the study is to observe andanalyze the correlation between percent liver weight and percent liver fat cells in control ratsand diabetic, insulin, metformin and insulin-metformin combination treated high fat diet (HFD)/ Streptozotocin (STZ) induced diabetic albino rats. Study Design: Experimental comparativestudy. Setting: Institute of Bio Medical Sciences (IBMS), Dow University of Health Sciences(DUHS), Ojha Campus, Karachi. Period: December 2014 to May 2015. Materials and Methods:The experimental study was conducted on 50 albino wistar rats. 10 rats served as control ratswhile rest of the rats were experimentally induced for diabetes type 2 and were then randomizedinto 5 groups. One group was treated with insulin, one with metformin, and the one groupwith insulin-metformin combination for 4 weeks. All the treated groups were compared withuntreated and control group. At the end of experiment, all the rats were sacrificed and liverswere isolated and weighed. Percent liver weight calculated. Liver cut sections were processedand stained to analyze the correlation in percent fat cells in liver percent liver weight and ineach treated and untreated diabetic groups, then the results were compared with control rats.Results: Data is analyzed by using SPSS Version 22. Pearson correlation was used to identifycorrelation between the percent liver weight and percent fat cells in liver of control, treated anduntreated diabetic groups. Significant and positive correlation (p-value 0.01) in insulin treatedgroup of diabetic rats was observed indicating that insulin has a role in causing fatty liver.Conclusion: Insulin treated diabetic group shows a significant positive correlation betweenpercent fat cells of liver and percent liver weight.