TO EVALUATE BACTERICIDAL ACTIVITY OF TABEBUIA IMPETIGINOSA AGAINST PSEUDOMONAS AERUGINOSA AND ITS SYNERGISTIC EFFECT WITH COMMON ANTIPSEUDOMONAS AERUGINOSA DRUG.

摘要

Objectives: In this research we assess in-vitro susceptibility of Pseudomonas aeruginosa (P. aeruginosa) using the ethanolic extract of medicinal plant Tabebuia impetiginosa (dried inner bark). To evaluate the synergistic effect of ethanolic extract of Tabebuia impetiginosa combine with ciprofloxacin In-vitro anti-Pseudomonas aeruginosa activities of the extracts and ciprofloxacin were confirmed, and synergism was verified for this combine extracts. Study Design: Experimental study. Period: October 2016 to February 2017. Place of Experiment: Rashid Latif Medical College, Lahore. Method: Diffusion method tests are mostly qualitative methods that are used to identify the antimicrobial activity, resistance and synergistic effect. The fresh plants inner bark was grinded and soaked in 95% ethanol for extraction. The antibacterial sensitivity of this compound against P. aeruginosa was assessed using the diffusion method. About 1000mg of grinded menstruum was added in 800ml of petroleum ether in a conical flask and adjust in rotary shaker at 100 rpm for 12 hours and then the final extract was filtered with 0.45mμ filter membrane and centrifuged at 2000rpm for 15 minutes. The final extract was redissolved in ciprofloxacin solution (10 for bioassay analysis. Results: We concluded that the fresh ethanolic extract of Tabebuia impetiginosa with ciprofloxacin has high antibacterial potency against P. aeruginosa which is prominent then a single. However this was not pure extract and if it is refined then it might gives significant antibacterial activity at low concentration. There is still need to test Tabebuia impetiginosa extract for antibacterial activity and to check synergistic effect with other drugs in-vivo against Pseudomonas aeruginosa. Extract presented the highest synergism rate with antimicrobial drug. Conclusion: In-vitro study showed Tabebuia impetiginosa fresh inner bark extract with ciprofloxacin dilution have significant antibacterial activity against Pseudomonas aeruginosa with P.value 0.001. Isolates were susceptible to this combine solution with mean zone diameter of 16.15 ± 0.95 mm and no regrowth was noticed. In the present research the synergistic effect of ciprofloxacin antibiotic with Tabebuia impetiginosa ethanolic extract were observe against Pseudomonas aeruginosa bacteria.