Isolation and Purification of Chemical Marker from Zingiber zerumbet Rhizome from Indonesia

摘要

A chemical marker from the rhizomes of Zingiber zerumbet was extracted, isolated and purified using methanol extraction andVacuum Liquid Chromatography (VLC) method. The sample was extracted with methanol and concentrated followed byfractionation with ethyl acetate and methanol. Ethyl acetate soluble fraction and methanol soluble fraction was subjected tosilica gel column chromatography to afford nine fractions. Gradient elutions were carried out using n-hexane-ethyl acetate (8:2,7:3, 6:4, 5:5, and 3:7 vol./vol.), ethyl acetate 100%, ethyl acetate-methanol (5:5 vol./vol.) and finally methanol 100%. Theisolated and purified chemical marker crystals were subjected to High Performance Liquid Chromatography (HPLC) to confirmthe purity. Purification of the chemical marker gave a white crystal with 97.96% purity and allegedly as 5-hydroxyzerumbone..