Zerumbone in Combination with Curcumin FOR Probable Treatment in Liver Cancer

摘要

There has been increasing evidence that cancers are dependent on a number of altered molecular pathways, which can developdiverse mechanisms of resistance to therapy with single agents. Therefore, combination regimens may provide the best hope foreffective therapies with durable effects. In this current investigation, the anti-cancer effect of zerumbone (ZER), a naturalcompound from Zingiber zerumbet smith of Zingiberaceae family and curcumin (a yellow pigment from Curcuma longa) wereused in combination for probable treatment in liver cancer. Both compounds were investigated in MTT cell proliferative assayas well as flow cytometric analysis (annexinV-FITC, cell cycle and TUNEL assay) on HepG2 cells in 3 ratios of ZER: CUR(1:3, 3:1 and 1:1). The IC50 obtained were 13μg/mL, 4 μg/mL and 7μg/mL respectively and the combinations further showed notoxic effect on normal liver cell lines (WRL68). The current findings revealed that the best combination of ZER: CUR is 3:1.The outcome of this study demonstrates that this combination has the ability to increased efficacy with limited toxicity in livercancer treatment.