Molecular Relationship and Biosynthetic Potential of Endophytic Bacteria

摘要

Endophytic bacteria live inside plant tissues without adverse effect to the host were isolated and identified using 16s rRNAanalysis by AIMST University’s researchers. From the 998 of isolated strain, Bacillus altiunidis, B.amyloliquefaciens, andB.thuringiensis are choosen for the study. A phylogenetic tree has been build in an attempt to identify possible relationshipbetween these endophytes using the neighbor joining method with 1000 bootstraps. However no cluster form between thebacteria either based on plant host family or geographical region of collection. Growing bacteria is done in 96 deep wellmicrotiter plates which is fast and cost effective. Three types of elicitors Fe2SO2, MgSO4, and Cu2SO4, were added to the growth medium to vary the growth conditions in the hope of producing different secondary metabolite. Solid phase extractionused to extract the secondary metabolites from bacterial cultures. The extraction method was modified from a standardoperating procedure that was developed for fungal metabolites. The secondary metabolite profiling was done using ultra highperformance liquid chromatography (UHPLC). The metabolites profiles were compared to identify variations in strain’smetabolomes. The structures of compound of interest will be elucidate using UV, capillary NMR and LC-MS.