Genetic Properties of Mycoplasma mycoides Subspecies mycoides LC Strains Isolated from a Goat in Japan in 1991

摘要

References(24) Field isolated Mycoplasma strains LGl and SFl were isolated from the lung and leg joint fluid of a goat, respectively in Japan in 1991. These strains were closely related and had the following biochemical properties: glucose was metabolized, arginine and urea were not hydrolyzed, tetrazolium was reduced, phosphatase produced, and casein and coagulated serum were digested. Serologically, these strains cross-reacted with three Mycoplasma species [M.mycoides subsp. mycoides (Mmm), M.mycoides subsp. capri, and M.sp.Group 7]. From the above results, the field isolates were considered to be Mmm. In addition, DNA-DNA relatedness between the field isolates and other Mycoplasma species called the "mycoides cluster" was determined, and a recently developed PCR method was also used for mycoplasmal identification. From these results, the field isolates were identified as Mmm LC type.