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首页> 外文期刊>The Journal of General and Applied Microbiology >SUBCELLULAR LOCALIZATION OF ADENYLATE CYCLASE DURING THE FRUITING BODY FORMATION IN COPRINUS MACRORHIZUS
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SUBCELLULAR LOCALIZATION OF ADENYLATE CYCLASE DURING THE FRUITING BODY FORMATION IN COPRINUS MACRORHIZUS

机译:马尾骨结实体形成过程中腺苷酸环化的亚细胞定位

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摘要

Adenylate cyclase from Coprinus macrorhizus was found in the particulate fractions containing plasma membrane, microsomes, and mitochondria. Glucose addition lead to inhibition of adenosine 3′:5′-cyclic monophosphate accumulation and fruiting body formation in vivo, but glucose did not inhibit the adenylate cyclase activity in vitro. The enzyme was solubilized by treatment with detergents or KCl. The localization of adenylate cyclase did not change in the course of fruiting body formation under various light conditions.
机译:在含有质膜,微粒体和线粒体的颗粒部分中发现了鬼伞鬼伞的腺苷酸环化酶。葡萄糖的添加导致体内抑制腺苷3':5'-环一磷酸的积累和子实体的形成,但是葡萄糖在体外并未抑制腺苷酸环化酶的活性。通过用去污剂或KCl处理使酶增溶。在各种光照条件下,子实体形成过程中腺苷酸环化酶的定位没有改变。

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