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Free and Total Plasma Cortisol Measured by Immunoassay and Mass Spectrometry Following ACTH1–24 Stimulation in the Assessment of Pituitary Patients

机译:在评估垂体患者的ACTH1–24刺激后,通过免疫测定和质谱法测定游离血浆和总血浆皮质醇

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Context:Measurement of plasma cortisol by immunoassay after ACTH_(1–24) stimulation is used to assess the hypothalamic-pituitary-adrenal (HPA) axis. Liquid chromatography-tandem mass spectrometry (LCMS) has greater analytical specificity than immunoassay and equilibrium dialysis allows measurement of free plasma cortisol.
机译:背景:ACTH_(1–24)刺激后,通过免疫测定法测定血浆皮质醇,用于评估下丘脑-垂体-肾上腺(HPA)轴。液相色谱-串联质谱(LCMS)具有比免疫测定更高的分析特异性,而平衡透析允许测量游离血浆皮质醇。

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