Modeling and Analysis of asymmetric Sieve shaped Skewed type microchannel network in BioMEMS for mass and size based mammalian cell separation and sorting using filtration method

摘要

In decades, there have been techniques which are combined with microfluidics and enforced to cell biology. It is based on microtechnology among biological terms to which some are regarded highly in cell separation technology and sorting of different types of cells in clinical fields, separating spherical as well as non-spherical cells and separation of blood cells. Employing a numerous physical principle, many separation methods for bioparticles were created which includes Deterministic lateral displacement (DLD), Pinched Flow fractionation (PFF), acoustic, Hydrodynamic, Dielectrophoresis (DEP), magnetic and Filtration methods. It is proposed and focused on the size-dependent bioparticles of mammalian cells (Klebsiella species) and blood cells with some advantages and disadvantages to get an exclusive idea about cell separation in microsystems. The proposed simulation and analysis of a microchannel having distinct angles (90o,15o,25o and 35o) with individual flow rates in microfluidics system for separation of bioparticles is been supported by a commercial software COMSOL Multiphysics. Where sieve shaped skewed type microchannel device consisting of 90o and 25o sieve structure provides a preferable separation through the targeted outlet-4 and outlet-1 of microchannel for a larger bioparticles and smaller particles (blood cells) through the other outlets of device