Chiral analysis by mass spectrometry using the kinetic method

摘要

Novel and quantitative mass spectrometry methods for rapid and accurate enantiomeric excess determination are presented. These methodologies use electrospray ionization (ESI) and mass spectrometry (MS) to detect and analyze, via collision-induced dissociation (CID), mass-selected transition metal complexes that promote enantio especific interactions. The data from CID are conveniently treated by the kinetic method, a sensitive linear free energy method of treating mass spectrometric results. Four different variations of this methodology are described: single ratio method (SR), quotient ratio method (QR), fixed ligand method (SRfixed), and quotient ratio method with fixed ligand (QRfixed). These individual methods are compared and their main features discussed in detail.