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Toxicological effect of ethanol on the development of chicken embryo in ova

机译:乙醇对卵子鸡胚发育的毒理作用

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This study aimed at finding out the toxicological effects of ethanol on chicken embryo during early stages of development. The nature and extent of toxicological effects were investigated to identify the concentration which causes the death of the embryo. The total (n=32) eggs were divided into four groups viz. control group, group A, group B and group C. Eight eggs were placed in each group for incubation. Different concentrations of ethanol were given to those groups viz. 5%, 10%, and 15% respectively. Gross anatomical defects particularly embryo’s body weight, body length, wings length and hind limbs length throughout seven days incubation were measured after the exposure of mentioned ethanol concentrations. The results of mean values of seven-day-old chick embryos exposed to 5% ethanol showed non-significant effect ( P 0.05) on body weight, wings length and the hind limbs length of all groups concerning control group chick embryo. But ethanol with a concentration of 10% and 15% showed a significant effect ( P 0.05) on the body length of chick embryo in comparison with 5% treated chicks. The results of hatchability showed that group A showed 90% normal hatchability at the 21SUPst/SUP day while group B showed no remarkable delay in comparison of group C which showed delayed hatching at 22SUPnd/SUP day due to exposure of heavy dose. Moreover, among the most frequent abnormalities, hind limb lengths at 15% and body weight at 5% were found reduced in size. Ethanol exposure increases the risks of developmental defects with increasing embryonic age, delayed and assisted hatching.
机译:这项研究旨在发现乙醇在发育早期对鸡胚的毒理作用。对毒理作用的性质和程度进行了研究,以确定引起胚胎死亡的浓度。将总共​​(n = 32)个卵分成四组。对照组,A组,B组和C组。每组放置八个卵进行孵化。将不同浓度的乙醇给予这些组。分别为5%,10%和15%。在暴露上述乙醇浓度后,​​测量了整个孵化7天的总体解剖缺陷,特别是胚胎的体重,体长,翅膀长度和后肢长度。与对照组鸡胚相比,暴露于5%乙醇中的7天大鸡胚的平均值对各组的体重,翅膀长度和后肢长度无显着影响(P> 0.05)。但是,与5%处理的雏鸡相比,浓度为10%和15%的乙醇对雏鸡胚的体长有显着影响(P <0.05)。孵化率结果显示,与C组相比,A组在第21天天孵化率达到90%,而B组则没有明显的延迟。 >由于暴露于大剂量而导致的一天。此外,在最常见的异常中,发现后肢长度减少15%,体重减少5%。乙醇暴露会随着胚胎年龄的增加,孵化的延迟和辅助而增加发育缺陷的风险。

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