Characterization of a Maize Sucrose-phosphate Synthase Protein and Its Effect on Carbon Partitioning in Transgenic Rice Plants

摘要

We obtained transgenic rice (Oryza sativa L. cv. Nipponbare) plants with the gene for maize sucrose-phosphate synthase (EC 2.4.1.14, SPS). Some of the transgenic plants over-expressed maize SPS (over-expressing plants) and some had reduced levels of native SPS protein (co-suppressed plants). There was a positive correlation between the amounts of maize SPS protein and SPS activities. However, apparent Km values for uridine diphosphoglucose (UDPG) were higher in over-expressing plants than in control rice plants. These results suggest that over-produced maize SPS protein was not fully activated. The sucrose contents did not differ significantly between control and over-expressing rice plants, but they were lower in co-suppressed plants than in control plants. The starch contents were negatively and the sucrose/starch ratios were positively correlated with SPS activities. Thus, carbon partitioning in the transgenic rice was changed, even though rice is predominantly a sucrose-former.