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首页> 外文期刊>Plant Biotechnology Journal >Genome?¢????wide survey of artificial mutations induced by ethyl methanesulfonate and gamma rays in tomato
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Genome?¢????wide survey of artificial mutations induced by ethyl methanesulfonate and gamma rays in tomato

机译:基因组广泛研究番茄中甲磺酸乙酯和γ射线诱导的人工突变

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摘要

Genome?¢????wide mutations induced by ethyl methanesulfonate (EMS) and gamma irradiation in the tomato Micro?¢????Tom genome were identified by a whole?¢????genome shotgun sequencing analysis to estimate the spectrum and distribution of whole?¢????genome DNA mutations and the frequency of deleterious mutations. A total of ~370????Gb of paired?¢????end reads for four EMS?¢????induced mutants and three gamma?¢????ray?¢????irradiated lines as well as a wild?¢????type line were obtained by next?¢????generation sequencing technology. Using bioinformatics analyses, we identified 5920 induced single nucleotide variations and insertion/deletion (indel) mutations. The predominant mutations in the EMS mutants were C/G to T/A transitions, while in the gamma?¢????ray mutants, C/G to T/A transitions, A/T to T/A transversions, A/T to G/C transitions and deletion mutations were equally common. Biases in the base composition flanking mutations differed between the mutagenesis types. Regarding the effects of the mutations on gene function, >90% of the mutations were located in intergenic regions, and only 0.2% were deleterious. In addition, we detected 1????140????687 spontaneous single nucleotide polymorphisms and indel polymorphisms in wild?¢????type Micro?¢????Tom lines. We also found copy number variation, deletions and insertions of chromosomal segments in both the mutant and wild?¢????type lines. The results provide helpful information not only for mutation research, but also for mutant screening methodology with reverse?¢????genetic approaches.
机译:通过全基因组shot弹枪测序分析鉴定了由甲磺酸乙酯(EMS)和γ射线诱导的番茄Micro-Tom基因组中的全基因组突变。基因组DNA突变的突变和分布以及有害突变的频率。共有〜370 Gb的配对配对末端读取四个EMS诱导的突变体和三个γ射线照射的线,分别为通过下一代测序技术获得了野生型和野生型。使用生物信息学分析,我们确定了5920个诱导的单核苷酸变异和插入/缺失(插入/缺失)突变。 EMS突变体中最主要的突变是C / G到T / A的转变,而γ-γ-ray突变体中,C / G到T / A的转变,A / T到T / A的转变,A /从T到G / C的过渡和缺失突变同样普遍。在诱变类型之间,碱基组成侧翼突变中的偏倚有所不同。关于突变对基因功能的影响,超过90%的突变位于基因间区域,只有0.2%的突变是有害的。另外,我们在野生型Micro TM的Tom系中​​检测到了1,140,687的自发单核苷酸多态性和插入缺失多态性。我们还发现突变型和野生型细胞系中染色体片段的拷贝数变异,缺失和插入。结果不仅为突变研究提供了有用的信息,而且为反向遗传方法的突变筛选方法提供了有用的信息。

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