Simple and Rapid Determination of Diuretics by Luminescent Method

摘要

Diuretics are drugs widely used in treatment of heart failure and hypertension and as doping agents in sports. Wrong prescription and excessive abuse can lead to negative side effects. Despite the effectiveness of methods usually used for the determination of diuretics (gas or liquid chromatography, capillary electrophoresis), they do not always provide necessary sensitivity. Moreover, sample preparation increases time of analysis. A rapid and sensitive luminescent method for determination of 5 diuretics (amiloride, bendroflumethiazide, bumetanide, furosemide, triamterene) in aqueous solutions and amiloride and triamterene in human urine is described. Intrinsic luminescent properties of protolytic forms of diuretics were studied in order to provide highly sensitive analysis. Investigation of interfering influence of diuretics was carried out to provide selective determination of triamterene, bumetanide and furosemide in aqueous mixtures of diuretics. Influence of urine at luminescent properties of diuretics was studied. The possibility of determination of triamterene and amiloride in human urine as individual substances and in mixture was proved. Simple and rapid technique for their determination in human urine was elaborated. The techniques elaborated for determination of triamterene in presence of other diuretics and furosemide in presence of commensurate amount of bumetanide allow enhancing specifity of analysis. Sufficient selectivity and sensitivity were reached in determination of amiloride and triamterene in human urine. The reduction of time of analysis due to avoiding sample preparation merits the techniques proposed.