Neuroprotective effects of 4,5-dimethoxypyrocatechol isolated from Cynanchum paniculatum on HT22 cells

摘要

Background:Cynanchum paniculatum, belongs to the family Asclepiadaceae and is used to treat various diseases, such as invigorate blood, alleviate edema and to relieve pain and toxicity for a long time.Materials and Methods:4,5-Dimethoxypyrocatechol was isolated from the 80% methanol extract of C. paniculatum and its neuroprotective effect was evaluated by MTT assay.Results:4,5-Dimethoxypyrocatechol had neuroprotective effect on the glutamate-induced cellular oxidative death in HT22 cells.Conclusion:Furthermore, we found that reactive oxygen species (ROS) accumulation and calcium concentration by oxidative stress were reduced by 4,5-dimethoxypyrocatechol in HT22 cells.