Determination of Paeonol in Cynanchum paniculatum by high Performance Capillary Electrophoresis

摘要

This design uses High Performance Capillary Electrophoresis (HPCE) to determine the Paeonol's content in Cynanchum paniculatum. Electrophoresis separation controlled conditions for uncoated fused silica capillary column (75 μm×52/60cm) injection height 7.5cm, and using a concentration of 40mmol/L borax solution as buffer. Using separate voltage 20KV to detect wavelength 270nm, temperature is 20°C, and the injection time control 10s. The Paeonol is linear relationship(r=0.998) within concentration of 3～400mg/L, and measuring 6 times its average recovery was 92.35%, RSD2.4%. After the contents were measured 7 times, Paeonol contain was 9.9mg/g RSD=6.6%. Conclusion for this design is that the method is simple, accurate and efficient with good reproducibility for the determination of paeonol in Cynanchum paniculatum determination.