Effects of insulin and the glucagon?￠????like peptide 1 receptor agonist liraglutide on the kidney proteome in db/db mice

摘要

Diabetes mellitus (DM) is a worldwide disease that affects 9% of the adult world population and type 2 DM accounts for 90% of those. A common consequence of DM is kidney complications, which could lead to kidney failure. We studied the potential effects of treatment with insulin and the glucagon?￠????like peptide 1 receptor (GLP?￠????1R) agonist liraglutide on the diabetic kidney proteome through the use of the db/db mouse model system and mass spectrometry (MS). Multivariate analyses revealed distinct effects of insulin and liraglutide on the db/db kidney proteome, which was seen on the protein levels of, for example, pterin?￠????4 ???± ?￠????carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor?￠????1 ???± (PCBD1), neural precursor cell expressed developmentally down?￠????regulated?￠????8 (NEDD8), transcription elongation factor?￠????B polypeptide?￠????1 (ELOC) and hepcidin (HEPC). Furthermore, the separation of the insulin, liraglutide and vehicle db/db mouse groups in multivariate analyses was not mainly related to the albumin excretion rate (AER) or the level of glycated hemoglobin A 1c (HbA 1c %) in the mice. In summary, we show that insulin and liraglutide give rise to separate protein profiles in the db/db mouse kidney.