The mictropropagation eficiensy by slowing the growth rate of Solanum tuberosum var. Gersa vitroplantlets

摘要

In plant ”living collection” for micropropagation optimization of potato local variety we used a slow growth technique, we used 2 systems: the double layer system (using a basal agarized medium and as supernatant a second layer with silicone oil or paraffin oil, or castor oil), and a normal agarized medium – by adding in culture media: manithol, sorbithol, AgNO3 or B9. The plantlets were vitroconservated 48 weeks in slow growth system; the control lot - during the 48 weeks - was subcultived 4 times: after 12, 24, 36 and 48 weeks of vitroculture. In 48 weeks of vitroplantlets slow growth did not reach the growth values which control lot achieved in 12 weeks of vitroculture – this showing the efficiency of the presented system. Silicone oil – as supernatant - is the best variant of slowing the growth in double layer system of Solanum tuberosum var. Gersa, B9 (diaminozide) had the highest inhibitory effect in normal agarized media.