Six dammarane-type saponins were extracted from steamed Panax notoginseng. Their chemical structures were identified spectroscopically as ginsenosides Rh1 (1), Rg1 (2), 20 (S)-Rg3 (3), 20 (R)-Rg3 (4), Rb3 (5), and Rb1 (6). Compounds (0.1-10 μM) were tested for inhibition of tumor necrosis factor-α (TNF)-induced nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) luciferase reporter activity using a human kidney 293T cell-based assay. Ginsenoside Rb3 (5) showed the most significant activity with an IC50 of 8.2 μM. This compound also inhibited the induction of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) messenger Ribonucleic acid (mRNA) in a dose-dependent manner after HepG2 cells had been treated with TNF-α (10 ng/mL).Keywords: Antitumor, dammarane-type ginsenoside, inhibitory activity, Panax notoginseng
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机译：从蒸三七提取了六种丹玛兰型皂苷。它们的化学结构经光谱鉴定为人参皂甙Rh1（1），Rg1（2），20（S）-Rg3（3），20（R）-Rg3（4），Rb3（5）和Rb1（6）。使用人肾293T细胞测试了化合物（0.1-10μM）对肿瘤坏死因子-α（TNF）诱导的核因子kappa轻链增强的激活B细胞（NF-κB）荧光素酶报道分子活性的抑制作用基于基础的分析。人参皂苷Rb3（5）表现出最显着的活性，IC50为8.2μM。在用TNF-α（10 ng / mL）处理HepG2细胞后，该化合物还以剂量依赖性方式抑制了环氧合酶2（COX-2）和诱导型一氧化氮合酶（iNOS）核糖核酸（mRNA）的诱导。关键词：抗肿瘤，达玛烷型人参皂苷，抑制活性，三七
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