Protective effects of Ampelopsis brevipedunculata against in vitro hepatic stellate cells system and thioacetamide-induced liver fibrosis rat model

摘要

Abstract Context: Ampelopsis brevipedunculata Maxim (Vitaceae) is a traditional medicinal herb used for treating liver disorders. Objective: The hepatoprotective effects of A. brevipedunculata ethanol extract (ABE) was investigated in experimental models of fibrosis. Materials and methods: Hepatic stellate cells (HSCs) system in vitro and thioacetamide (TAA)-induced liver fibrosis rat model in vivo were used. Sprague–Dawley rats were divided into five groups of eight each (control, TAA, TAA with ABE 10?mg/kg, ABE 100?mg/kg and silymarin 50?mg/kg groups, respectively). Fibrosis was induced except to the control group by TAA (200?mg/kg, i.p.) twice per week for 13?weeks. ABE and silymarin was administered orally six times per week from the 7th week to the 13th week. Results: In HSC-T6 cells, ABE (0.1?mg/mL) and silymarin (0.05?mg/mL) significantly (p?p?in vivo studies, ABE (10 and 100?mg/kg) treatment ameliorated the altered levels of serum biomarkers significantly (p?p?p?