METHOD FOR PREPARING QUIESCENT HEPATIC STELLATE CELL, AND MODEL FOR EVALUATION OF ACTIVATION OF QUIESCENT HEPATIC STELLATE CELL

摘要

The present invention addresses the problem of providing: a method for preparing a quiescent hepatic stellate cell by inducing the differentiation of a pluripotent stem cell; and a method for screening for a chemical substance involved in the activation of a quiescent hepatic stellate cell. According to the present invention, a method for preparing a quiescent hepatic stellate cell is provided, which comprises a step of inducing the differentiation of an iPS cell and fractionating a quiescent hepatic stellate cell by employing a NGFR-positive phenotype as an index. Also provided is a screening method for selecting as a candidate substance that can be used as a drug for treating or preventing a fibrosis-related liver disease on the basis of the expression amount of a gene marker that is expressed specifically in a hepatic stellate cell that is activated by introducing a reporter gene into the quiescent hepatic stellate cell.