Antioxidant and Cytoprotective Properties of Three Egyptian CyperusSpecies Using Cell-free and Cell-based Assays

摘要

Three Egyptian Cyperus species viz. Cyperus esculentus, C. rotundus, and C. papyrus that belong to Cyperaceaewere used as food and medicines since ancient times. This study aimed to investigate the direct and indirect antioxidantproperties of the selected species in two chemical assays (FRAP and DPPH) and a cell-based bioassay in hepa1c1c7 cellsusing t-butyl hydroperoxide (TBHP) as the inducer of cytotoxicity. Three different extracts were prepared from eachCyperus tuber. The results indicated that the 80% EtOH extract of C. papyrus was the most potent with direct antioxidantactivity in both DPPH (EC50: 5.1 μg/ml) and FRAP (FE: 48.7 μg/ml) assays and a significant full cytoprotection againstTBHP at 100 μg/ml.