Evaluation of Natural Substances’ Protective Effects Against Oxidative Stress in a Newly Developed Canine Endothelial Cell-Based Assay and in Cell-Free Radical Scavenging Assays

摘要

Oxidative stress has been shown to induce damage in a variety of canine and human cardiovascular cell types, and is implicated in the initiation and progression of various cardiac diseases. Various antioxidant agents used in the management of heart failure appear to confer cardio-protective benefits. In hght of growing evidence for the inadequacy of a monodimensional antioxidant strategy, there is a demand for an integrated approach using a combination of antioxidant compounds with complementary or interdependent effects. In addition there is a lack of specific data on the effects of natural antioxidant substances in canine endothelial cells. To address this topic, we developed a new canine aortic endothelial cell-based H2O2 oxidative stress assay.The antioxidant and cytoprotective effects of four substances, ie, L-camitine, taurine, pomegranate extract, and soy isoflavone extract, alone or in combination, were investigated with the newly developed cell-based assay and two free radical scavengingassays. The results show that pomegranate extract, both alone and in combination with the other substances, possesses significant strong antioxidant and cytoprotective activities in canine endothelial cells. Data from this and other published studies suggest that these natural substances could be of interest when developing a multi-dimensional dietary strategy to reduce the onset and progression of oxidative stress-induced canine disease and perhaps more specifically the endothelial degeneration involved in progressive valvular insufficiencies.