Actin in spermatids and spermatozoa of Teladorsagia circumcincta and Trichostrongylus colubriformis (Nematoda, Trichostrongylida)

摘要

Trichostrongyle nematodes provide valuable models for the study of nematode sperm, because their male germ cells are large and elongate, allowing easy identification of cell organelles. A previous study led to the unexpected result that actin co-localizes with MSP (Major Sperm Protein) in spermatids and spermatozoa of Heligmosomoides polygyrus. Actin expression in male germ cells of Teladorsagia circumcincta and Trichostrongylus colubriformis was studied using a monoclonal anti-actin antibody. Actin was demonstrated in the ? fibrous bodies ? in spermatids and in an anterior cap in spermatozoa. The actin labelling pattern in the two species studied was similar to that found in H. polygyrus, suggesting that this distribution of actin might be general for nematode male germ cells.