Production of microbial biomass protein by sequential culture fermentation of Arachniotus sp., and Candida utilis

摘要

Sequential culture fermentation by Arachniotus sp. at 35oC for 72 h and followed by Candida utilis fermentation at 35oC for 72 h more resulted in higher production of microbial biomass protein. 6% (w/v) corn stover, 0.0075% CaCl2.2H2O, 0.005% MgSO4.7H2O, 0.01% KH2PO4, C: N ratio of 30:1 and 1% molasses gave higher microbial biomass protein production by the sequential culture fermentation of Arachniotus sp., and C. utilis. The mixed microbial biomass protein produced in the 75-L fermentor contained 16.41%, 23.51%, 10.9%, 12.11% and 0.12% true protein, crude protein, crude fiber, ash and RNA content, respectively. The amino acid profile of final mixed microbial biomass protein showed that it was enriched with essential amino acids. Thus, the potential utilization of corn stover can minimize the cost for growth of these microorganisms and enhance microbial biomass protein production by sequential culture fermentation.