Isolation and molecular identification of a facultatively anaerobic bacterium from the hot spring of Azad Kashmir

摘要

A thermophilic facultatively anaerobic bacterium (TP-1) was isolated from the Tatta Pani hot spring in Azad Kashmir. The isolate had entire and slimy colonies while the cells were small rods and gram-positive. Phylogenetic analysis based on partial 16S rRNA gene sequence comparisons showed high levels of similarity of the TP-1 ( 95%) with Geobacillus pallidus. Optimum temperature and pH for the growth of the strain TP-1 were found to be 65oC and 7.0 respectively. TP-1 gave positive results for ortho Nitrophenyl-β-D-Galactopyranosidase (ONPG) and Gelatin hydrolysis (GEL) while other tests such as Arginine dihydrolases, Lysine decarboxlase, Ornithine decarboxylases, H2S production, Urease, Tryptophan deaminases, Indole production, Acetoin production, Fermentation/oxidation (Glucose, Mannitol, Inositol, Sorbitol, Rhamnose, Sucrose, Melibiose, Amygdalin, Arabinose) and Citrate utilization were negative.