Tricholomopsis flammula Métrod ex Holec (Basidiomycota, Agaricales)-an addition to the mushroom flora of Pakistan based on molecular identification

摘要

A species of Tricholomopsis was collected from Himalayan moist temperate forest of Pakistan and identified on the basis of morphological and molecular characterization. The target region of rDNA (ITS1 5.8S ITS2) was amplified using universal fungus primers ITS1 and ITS4. Its rDNA sequence, when aligned in GenBank by performing BLAST, matches 100% with Tricholomopsis flammula. The rDNA sequence of this species forms a distinct clade from the rest of species of the same genus. This species is being reported first time from Pakistan.