Detection of phytoplasma from diseased potato samples

摘要

The stunted and deformed potato (Solanum tuberosum) samples were received from Potato Research Station, Sahiwal at Crop Diseases Research Program, National Agriculture Research Centre, Islamabad for diognosis of the disease. The symptoms of little leaf, stunted growth and deshaped tubers were observed in the sample which was assumed as infected by Phytoplasma. Polymerase Chain Reaction (PCR) technique was selected for identification of the pathogen. An amplified fragment of 550 base pair was obtained which confirmed the presence of phytoplasmal pathogen in infected tubers and plant.