Insulin producing cell clusters derived from very small embryonic like stem cells are potent to treat diabetics mice

摘要

Considerable effort has been expended to isolate stem cells from a number of different tissues to use in regenerative medicine. In this study, we purposed to reckon up the ability of mouse very small embryonic like stem cells (VSEL) differentiation into insulin producing cell clusters(IPCCs), in order to treatment of diabetes. Mouse Bone marrow VSEL stem cells, isolated with Fluorescent activating cell sorting (FACS) method by using Fluorescent antibodies against CD45, CXCR4 and Sca1markers. After differentiation of VSEL stem cells into IPCCs with Activin A and nicotinamide as main factors of differentiation, RT-PCR and immunocytochemistry revealed that newly IPCCs could express Ins1, Ins2, PDX1 and Glut2. Invitro insulin secretion by IPCCs was confirmed by ELISA kit. For treatment, we injected IPCCs into thigh muscle of diabetic mice. Results showed that newly derived IPCCs were capable to produce insulin in vivo. There was a significant decrease in mean blood sugar of diabetic mice after passing 3 weeks from cell therapy. This study provides a strategy for using autologous VSEL stem cells for curing diabetes and other regenerative disease, as an alternative for other stem cell types.