In – Vitro activity of Desmostachya bipinnata (L.) Stapf successive extracts against Helicobacter pylori clinical isolates

摘要

Helicobacter pylori are well acknowledged as a major cause of gastrointestinal ailments and gastric cancers. Therefore, the present study aimed to investigate the potential in vitro activity of Desmostachya bipinnata against H. pylori , focusing on the determination of the most active extract responsible for the anti-helicobacter activity to produce new active drug from natural source. Desmostachya bipinnata total alcohol and successive extracts were in vitro tested against H. pylori . All extracts showed promising anti Helicobacter pylori activities. The most effective extract was diethyl ether extract, it showed 75% growth inhibition of the clinical Isolates bacterial Helicobacter pylori , in addition it showed high count reduction on the selected organisms in the different concentrations used (2xMIC, MIC & ? MIC) compared with the untreated controls as well as the other extracts (chloroform, ethyl acetate and n-butanol). The oral median lethal dose (LD50) of the alcohol extract of the plant by doses up to 5000?mg/kg didn’t showed any mortality or morbidity, in addition no side effects were recorded on both liver and kidney functions this means that the extract was safe for use.