A NEW SIMPLIFIED RADIOISOTOPE METHOD FOR DETERMINING HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE AND ADENINE PHOSPHORIBOSYLTRANSFERASE WITH THE USE OF THIN-LAYER RADIO-CHROMATOGRAM SCANNER

摘要

An electrophoretic method for determining hyppoxanthine-guanine and adenine phosphoribosyltransferase in a mammalian tissue is presented. The method based on Kizaki's micro-assay procedure was improved with modification using a technic of thin-layer radio-chromatographic scanning. The modification serves to reduce the time in the enzyme assay without any interference among nucleotide, nucleoside and other decomposition product. In this paper, the assay was applied to crude extract of cow snout epidermis, and the salvage enzyme activity in the epidermis was determined.