Molecular characterization of Grey Steppe cattle breed for its genetic biopreservation

摘要

The Romanian grey steppe breed is subject to a genetic conservation program, this being the reason why these studies have been made. In Tf position was identified 6 types of genotypes: AA, AD, AE, DD and EE. The largest registered frequency was for the homozygous genotype DD with 37.21% (10 individuals) and the heterozygous genotype AD with 26.14% (9 individuals). The milk protein’s polymorphous system study showed the six major types of proteins: alpha casein S, (aSl-cz), beta casein (B-cz), kappa casein (K-cz), beta-lactoglobulin (B-lg), alpha-lactalbumin (a-la) and alpha-casein S, (aS2-cz). A new allele gene aS1-casein I?’, was identified at the as)-casein location, for 2 of the total number of analysed individuals. It has the form of a band with its isoelectric point situated between allele C and B, closer to allele C. This ancient particular allele proves its antiquity and it is the first of its kind among the Podolice family. Thereafter, this new casein can be an important genetic marker that shows the breed’s origins. In the grey steppe breed genetic amelioration and conservation process the techniques regarding genetic and molecular markers are very useful as well as other techniques and biotechnology such as: semen material conservation, super-ovulation and egg cells conservation.