IN VITRO activity of β-amirinic phytocompounds – a basic screening for breast cancer therapy

摘要

As a result of biotechnological research concerning the indigene phytotherapeutic potential follow-up, we obtained two f-amirinic compounds (GSO-1 and GSO-2) with antitumorous indications. In order to define the specific target action of these compounds, we developed an “in vitro” experimental model for antiproliferative status evaluation on adherent, epithelial type breast adenocarcinoma (MCF-7 cell line). The biocompounds were tested at different concentrations, at 24 and 48h cell culture treatment, as a screening of most important parameters for antitumorous activity (cell proliferation, DNA synthesis, apoptosis). The target parameters were obtained by cellular analysis methods as microscopy and flow cytometry, using competitive software. The results marked a significant and differentiated activity of GSO-1 and GSO-2. The first one is a relevant apoptosis inducer, but the second compound acts as an inhibitor for MCF-7 cells proliferation and DNA synthesis.