Prevalence of steroid hormone residues by GC-MS/MS screening in animal matrices in Romania

摘要

The aim of this one year survey study was to assess, the prevalence of steroid hormones residues in theaim of National Residue Control Plan. GC-MS/MS in urine, serum, liver and muscle tissue in conformity withSOP ARO/492-2006, respectively SOP ARO/534-2008 was performed on 273 samples. Samples were purified using a one-step cleaning, detection being performed to a Varian 320-MS GC-MS/MS spectro-photometer (able to identify and confirm the matrix presence at 0.5 μg.kg-1 or 0.5 μg.L-1), the CCα values for most compounds being between 0.1-0.3 μg.kg-1 or μg.L-1. The percentage of analyzed samples/species was: 40.29% poultry; 34.43%, cattle; 22.71% swine; 1.43% sheep and goats and respectively 1.09% equines. From total number of samples analyzed for detection of natural or synthetic steroid hormones / category, considered in this study, regardless of the matrix (urine, serum, and muscle tissue), 0.36% of samples were for medroxyprogesterone, 12.09% for estradiol, 31.5% for nortestosterone, respectively 56.05% for trenbolone. Samples analyzed / animal specieswere compliant for: medroxyprogesterone, estradiol, testosterone and trenbolone-cloud. In the case of estradiol, values identified were between 0.01-0.03 ppb for β fraction and of 0.01-0.06 ppb, for α fraction, but below detection limit of the method used.