Molecular characterization of virulence and resistance features in Staphylococcus aureus clinical strains isolated from cutaneous lesions in patients with drug adverse reactions

摘要

Patients treated with epidermal growth factor inhibitors often experience cutaneous adverse reactions that could be complicated by infections driven by specific pathogens such as the community emergent methicillin resistant Staphylococcus aureus. This study was conducted on a total of 42 S. aureus clinical strains isolated in 2016 from acneiform reactions pustule and periungual lesions in patients with cutaneous drug adverse reactions. Multiplex PCR was performed on genomic DNA from isolates in order to identify the SCCmec central elements and the virulence genes: bbp (bone bound sialoprotein), ebpS (elastin-binding protein), fnbB, fnbA (fibronectin-binding proteins), fib, clfA, clfB (clumping factors A and B), cna (collagen-binding protein), luk-PV (Panton-Valentine leukocidin), hlg (gamma-haemolysin), tst (toxic shock syndrome toxin). The obtained results confirmed the increased virulence potential and the high prevalence of SSCmec type VIII, followed by type IV and II in cutaneous isolates from patients with dermatologic toxic effects.