Early screening of dentin caries using the methods of Micro-Raman and laser-induced fluorescence spectroscopy

摘要

The work presents microspectroscopic studies of the caries development and phase formation in dentin tissues using the methods of laser-induced fluorescence (LIF) and Raman spectroscopy. The results show that the data obtained near the boundary of intact and infected dentin by these two methods under simultaneous scanning from micro-regions confirm and complement each other. The analysis of the LIF spectra proves that the detected bands in Raman spectroscopy can be referred to the porphyrins, amino acids and DNA/RNA of microorganisms. In the laser-induced fluorescence spectra this fact appears in the “red” region of a fluorescence band of 650–716?nm. The fundamental differences that were found in the spectra are markers of pathologies in dentin tissues of human teeth during the development of caries and they can be employed for methods of in the early diagnostics.